MCI BUFFER™ for Amino Acid Analysis

MCI BUFFER™ kits, PH-KIT and PF-KIT are composed of sodium citrate buffer and lithium citrate buffer, respectively.
Both of these buffer kits contain the necessaries for the analysis of amino acids in protein hydrolyzates or in physiological fluids with MODEL LA-8080, L-8900, L-8800(A), L-8500(A) HITACHI High Speed Amino Acid Analyzer.
They are ready to use for analysis without further dilution or fine adjustment of their pH values.

MCI BUFFER™ Product Lineup

Code No. Product Name Contents Volume Remarks
2-503-00 PH-KIT PH-1 1L×2 1st buffer
PH-2 1L×1 2nd buffer
PH-3 1L×1 3rd buffer
PH-4 1L×2 4th buffer
PH-RG 1L×1 Regeneration
2-504-00 PF-KIT PF-1 1L×2 1st buffer
PF-2 1L×1 2nd buffer
PF-3 1L×1 3rd buffer
PF-4 1L×2 4th buffer
PF-RG 1L×1 Regeneration

Each single item in the kits is also available.

STANDARD ANALYSIS (PH)

Apparatus
Model L-8500 HITACHI High Speed Amino Acid Analyzer

Analytical Condition
Column: HITACHI Ion-Exchange column ♯2622SC-PH
Pre-column: HITACHI Packed column ♯2650L
Eluent: MCI BUFFERTM PH-KIT
Sample: Standard Calibration Mixture for Protein Hydrolyzate
Detection: Ninhydrin

PHYSIOLOGICAL FLUID ANALYSIS (PF)

Apparatus
Model L-8500 HITACHI High Speed Amino Acid Analyzer

Analytical Condition
Column: HITACHI Ion-Exchange column ♯2622SC-PF
Pre-column: HITACHI Packed column ♯2650L
Eluent: MCI BUFFERTM PF-KIT
Sample: Standard Calibration Mixture for Physiological Fluid
Detection: Ninhydrin

Precautions for Use

- Keep cool, dark and free from ammonia.

- Supply whole content of buffers in these kits to the reservoirs of HITACHI High Speed Amino Acid Analyzer without dilution.

-For a Model L-8500A HITACHI High Speed Amino Acid Analyzer, the nitrogen-gas pressure applied to the buffer containers should be in the range of from 0.35 to 0.4 kgf/cm2, or from 0.034 to 0.04MPa.

- Use before the effective date described on the carton case. Use up each solution promptly, as the solutions are subject to change in quality once the bottles are opened.

- Both kits contain adequate amounts of buffers necessary for the continuous operation of Model L-8800, Model L-8500A, Model L-8500 HITACHI High Speed Amino Acid Analyzer for around twenty days under the standard operating conditions.

- Refer to the instruction manual of HITACHI High Speed Amino Acid Analyzer for the details of the operation.