MCI BUFFER™ for Amino Acid Analysis

MCI BUFFER™ kits, PH-KIT and PF-KIT are composed of sodium citrate buffer and lithium citrate buffer, respectively.
Both of these buffer kits contain the necessaries for the analysis of amino acids in protein hydrolyzates or in physiological fluids with MODEL LA-8080, L-8900, L-8800(A), L-8500(A) HITACHI High Speed Amino Acid Analyzer.
They are ready to use for analysis without further dilution or fine adjustment of their pH values.

MCI BUFFER™ Product Lineup

Code No. Product Name Contents Volume Remarks
2-503-00 PH-KIT PH-1 1L×2 1st buffer
PH-2 1L×1 2nd buffer
PH-3 1L×1 3rd buffer
PH-4 1L×2 4th buffer
PH-RG 1L×1 Regeneration
2-504-00 PF-KIT PF-1 1L×2 1st buffer
PF-2 1L×1 2nd buffer
PF-3 1L×1 3rd buffer
PF-4 1L×2 4th buffer
PF-RG 1L×1 Regeneration

Each single item in the kits is also available.

STANDARD ANALYSIS (PH)

Apparatus
Model L-8500 HITACHI High Speed Amino Acid Analyzer

Analytical Condition
Column: HITACHI Ion-Exchange column ♯2622SC-PH
Pre-column: HITACHI Packed column ♯2650L
Eluent: MCI BUFFERTM PH-KIT
Sample: Standard Calibration Mixture for Protein Hydrolyzate
Detection: Ninhydrin

PHYSIOLOGICAL FLUID ANALYSIS (PF)

Apparatus
Model L-8500 HITACHI High Speed Amino Acid Analyzer

Analytical Condition
Column: HITACHI Ion-Exchange column ♯2622SC-PF
Pre-column: HITACHI Packed column ♯2650L
Eluent: MCI BUFFERTM PF-KIT
Sample: Standard Calibration Mixture for Physiological Fluid
Detection: Ninhydrin