CRS_Chromatogram_E

Application of MCI GEL HPLC columns

Separation of isoleucine isomers on MCI GEL CRS10W column


Eluent	2 mM CuSO4
flow rate	0.5 mL/min
detection	UV 254 nm

Separation of six amino acids on MCI GEL CRS10W column


eluent	1 mM CuSO4
flow rate	1.0 mL/min
detection	UV 254 nm

For each pair of DL isomers, D-form isomer elutes faster.

Separation of 2-hydroxycarboxylic acids on CRS10W column


	eluent flow rate detection	2 mM CuSO₄ : CH₃CN= 9 : 1 1.0 mL/min UV 254 nm
	1,2 = D-, L-lactic acid 3,4 = D-, L-2-hydroxybutyric acid 5,6 = D-, L-a-hydroxyvaleric acid 7,8 = D-, L-a-hydroxyisocaproic acid

Separation on MCI GEL CRS10W and CRS15W

Separation of DL-alanine


eluent	0.1 mM CuSO4
flow rate	0.5 mL/min
detection	UV 254 nm

Separation of D-lactic acid (50 ppm)


eluent	2 mM CuSO4
flow rate	0.5 mL/min
detectoin	UV 254 nm

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Last updated: 3 October 2000. ©copyright Mitsubishi Chemical Corporation