Application of MCI GEL HPLC columns
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Green_Bead.gif (973 ???) Separation of amino acids on  MCI GEL CK10M column
CK10U_AminoAcids.jpg (23248 ???)
Conditions
eluent 


 

A 0.2 N citrate pH 3.15
B 0.2 N citrate pH 3.20
C 0.2 N citrate pH 4.20
D 0.2 N citrate pH 9.70

flow rate

0.5 mL/min
temp

55 ºC
detection ninhydrin deriv


It is recommended to use a precolumn, MCI GEL AFR2PC, for ammonium ion removal in amino acid analysis with a cation exchange resin, MCI GEL CK10U etc.

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Last updated: 3 October 2000.  ©copyright Mitsubishi Chemical Corporation